Isolation and characterization of variants of Bombyx mori nuclear polyhedrosis virus.
نویسندگان
چکیده
منابع مشابه
Cytoplasmic Polyhedrosis Virus of Bombyx mori
Two structurally different classes of oligonucleotides accumulate in vitro in cytoplasmic polyhedrosis virus (CPV) transcription mixtures in molar excess as compared to the completed RNA products. The first class consists of oligonucleotides which correspond to the 5’terminal sequence of the virus mRNAs (referred to as initiator oligonucleotides). The major species of initiator oligonucleotides...
متن کاملIdentification and characterization of the p35 gene of Bombyx mori nuclear polyhedrosis virus that prevents virus-induced apoptosis.
Nucleotide sequence analysis of the Bombyx mori nuclear polyhedrosis virus (BmNPV) genome revealed the existence of a gene homologous to the p35 gene of Autographa californica NPV (AcNPV), which has been shown to prevent virus-induced apoptosis. The BmNPV p35 gene showed 96.1% nucleotide and 89.6% predicted amino acid sequence identity to the AcNPV p35 gene. A mutant BmNPV (BmP35Z) lacking a fu...
متن کاملMolecular cloning and physical mapping of the genome of Bombyx mori nuclear polyhedrosis virus.
A restriction fragment library which covered the entire genome of Bombyx mori nuclear polyhedrosis virus (BmNPV) was constructed using plasmid vectors. By analysis of cloned and viral DNA by double digestion with endonucleases and by hybridization techniques, a complete physical map of BmNPV was constructed for BamHI, EcoRI, HindIII, KpnI, PstI and SmaI. Five regions of repeated sequences conta...
متن کاملDivergence and evolution of homologous regions of Bombyx mori nuclear polyhedrosis virus.
Homologous regions (hrs) (hr1,hr2-left,hr2-right,hr3,hr4-left,hr 4-right, and hr5) similar to those found in the Autographa californica nuclear polyhedrosis virus (AcNPV) genome were found in the Bombyx mori NPV (BmNPV) genome. The BmNPV hrs contained two to eight repeats of a homologous nucleotide sequence which were on average about 75 bp long. All of these homologous sequence repeats contain...
متن کاملThe p95 gene of Bombyx mori nuclear polyhedrosis virus: temporal expression and functional properties.
As part of our effort to identify baculovirus proteins acting as transcriptional regulators, we have characterized a gene, p95, of Bombyx mori nuclear polyhedrosis virus (BmNPV) that encompasses an open reading frame for a putative 95-kDa polypeptide (P95). The N-terminal half of the conceptually translated P95 contains two zinc finger-type DNA-binding motifs, and its C terminus contains a prol...
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ژورنال
عنوان ژورنال: Japanese journal of applied entomology and zoology
سال: 1990
ISSN: 0021-4914,1347-6068
DOI: 10.1303/jjaez.34.279